Sureselect Human All Exon V7

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Sleek Design, Best-in-Class Coverage, Minimal Sequencing

The Agilent SureSelect Human All Exon V7 delivers unmatched coverage of targeted regions with minimal sequencing. Designed using the GRCh38/hg38 genome assembly, this exome design targets only coding regions from RefSeq, CCDS, GENCODE &USC Known Genes. all exon sequencing product features a novel bait design algorithm resulting in an end-to-end design size of only 48.2 MB. A novel bait design algorithm results in a sleek design with an end-to-end design size of only 48.2Mb.

The exome sequencing system provides excellent uniformity and allows more samples to be pooled per flow cell, for more cost-effective sequencing. The accurate targeting ensures that even hard-to-capture exons can be accessed. In addition, the system is compatible with all SureSelect Library prep solutions, making it one of the most versatile exome solutions for a wide range of sample types including low input material and FFPE samples, and workflow needs such as enzymatic or mechanical shearing.

Features

With just 5.3 GB of sequencing, the SureSelect Human All Exon V7 provides 20x coverage of >94% of targeted bases.
Excellent overall uniformity and percent on-target enable variant calling with high confidence while reducing sequencing costs.
Targets protein coding regions from RefSeq, GENCODE, CCDS and UCSC known genes, including hard-to-capture exons that are omitted from other exomes.
Targets all pathogenic variants in the genes included in the ACMG guidelines for secondary findings.
The raw data generated from human all exon sequencing can be readily analyzed using the Agilent Alissa Clinical Informatics Software, solving the data analysis bottleneck.

Sureselect XT Low Input Reagent Kit

The SureSelect^XT Low Input Reagent Kits are low input DNA library prep kits with hybrid-capture-based target enrichment, optimized for low input FFPE samples. These kits include reagents for library prep, hybridization, and capture. They can be used with any SureSelect custom catalog panel or exome.

Generate libraries from as little as 10 ng of input from intact or highly fragmented FFPE DNA
Get up to 192 sample indexes in the 96-well plate format, enabling automation for greater sequencing application flexibility
Enable 96 unique dual sample indexes (optional) to reduce index hopping
Use optional molecular barcodes (MBC) to remove false positives and accurately detect variants
Streamline your workflow with a 90-minute hybridization and master-mixed reagents
Transform samples into sequencing-ready libraries in 8 hours for a faster, more efficient workflow
Use with SureSelect exomes, or catalog panels or easily create custom panels with the SureDesign software application
Optional enzymatic fragmentation module for more flexibility